| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lactobacillus crispatus | AGGCGTTGGCTTTCGTTG | TGGGCAAATCCAGATGCAGT | 58.97 | 59.96 | 165 | 68 |
100.00%
|
42.86%
|
| Primer 2 | Lactobacillus crispatus | AAGGCGTTGGCTTTCGTTG | TGGGCAAATCCAGATGCAGT | 59.64 | 59.96 | 166 | 68 |
100.00%
|
42.86%
|
| Primer 3 | Lactobacillus crispatus | GCGTTGGCTTTCGTTGGTC | TGGGCAAATCCAGATGCAGT | 60.37 | 59.96 | 163 | 68 |
100.00%
|
42.86%
|
| Primer 4 | Lactobacillus crispatus | TCGTTGGTCAACCATGGCA | TGGGCAAATCCAGATGCAGT | 59.85 | 59.96 | 153 | 63 |
100.00%
|
42.86%
|
| Primer 5 | Lactobacillus crispatus | TGGTTCGCAAAGACGACAAC | TGTTGAGCCGAAGTTGCAG | 59.35 | 58.68 | 153 | 60 |
100.00%
|
57.14%
|
| Primer 6 | Lactobacillus crispatus | TCTGCTGATCGTGATTGATGC | TGGCGAGACATCAATTCCAGT | 58.79 | 59.72 | 160 | 59 |
100.00%
|
57.14%
|
| Primer 7 | Lactobacillus crispatus | CTGCTGATCGTGATTGATGCA | TGGCGAGACATCAATTCCAGT | 59.06 | 59.72 | 159 | 57 |
100.00%
|
57.14%
|
| Primer 8 | Lactobacillus crispatus | GCGTTGGCTTTCGTTGGT | TGGGCAAATCCAGATGCAGT | 59.28 | 59.96 | 163 | 56 |
100.00%
|
42.86%
|
| Primer 9 | Lactobacillus crispatus | GGGATGTGCTACAGCGTGT | AGCCCATCCACAATACAGGC | 60.08 | 60.11 | 286 | 55 |
100.00%
|
42.86%
|
| Primer 10 | Lactobacillus crispatus | AGCACGAACGATTGAGGCA | AGCCCATTTGTCAGCCCA | 60.01 | 59.15 | 187 | 54 |
100.00%
|
28.57%
|